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KMID : 0613820080180111485
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Journal of Life Science
2008 Volume.18 No. 11 p.1485 ~ p.1492
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Prostaglandin A₂-induced Apoptosis is Not Inhibited by Heme Oygenase-1 in U2OS Cells
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Ko Kyoung-Won
Lee Sun-Young
Ahn Ji-Hyun
Kim Jae-Taek
Kim In-Kyung
Kim Ho-Shik
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Abstract
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Prostaglandin A₂ (PGA₂), one of cyclopentenone PGs, induced both apoptosis and heme oxygenase (HO)-1 expression in U2OS cells. PGA₂-induced apoptosis was not perturbed by either over-expression or knock-down of HO-1, whereas H₂O₂-induced cell death was inversely modulated by the expression level of HO-1. In addition, N-acetyl-L-cysteine (NAC), a thiol antioxidant, blocked both apoptosis and HO-1 expression induced by PGA₂. But, non-thiol antioxidants like butylated hydorxyanisole (BHA) and ascorbic acid did not block either apoptosis or HO-1-induction. Taken together, these results suggest that PGA₂ induces both apoptosis and HO-1 expression, which are critically related to the thiol-reactivity of PGA₂, but not oxidative stress, and HO-1 expression may be independent or functionally located downstream of apoptosis by PGA₂ without contribution to apoptosis progression.
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KEYWORD
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Prostaglandin, cyclopentenone, apoptosis, heme oxygenase-1, oxidative stress
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